Assay Development and High-Throughput Screening
The goal of the NCTT Assay Biology Teams is to optimize biochemical, cellular and model organism-based assays requested or submitted by the biomedical research community for high-throughput small molecule screening. The results of these screens (probes) can be used to further interrogate protein and cell functions and biological processes relevant to physiology and disease. In addition, these probes can be further optimized to become potential lead candidates in the drug development pipeline.
The NCTT Assay Guidance Manual and the High Throughput Screening Assay Guidance Criteria contain detailed information on developing appropriate assays for high-throughput screening.
Assay development and high-throughput screening occurs in several programs within NCTT:
Assay Development and High-Throughput Screening Technologies Group
The NCTT Technology Development Program is in the Chemical Genomics Branch. It supports novel assay development for projects throughout the Center.Tox21
Assay development in the Tox21 program supports novel assays to interrogate biological systems to discovery and understand toxic effects of selected compounds.Probe Development Branch
The three Teams within the Probe Development Branch actively work with investigators through the Molecular Libraries Program to enhance their assays for high-throughput screening.Therapeutic Development Branch
The Biology group in the Therapeutic Development Branch develops assays for secondary screening of candidate drug compounds under development.
Last Updated: September 6, 2011
